Diomics has developed an industry-disrupting polymer for recovery of DNA from biological samples for diagnostics, forensics & biohazard detection applications. Our DiomatTM polymer can be used to produce swabs, kits and collection devices for efficient and automated recovery of DNA from blood, urine & buccal samples. Unlike cotton or rayon used in most collection systems today, DiomatTM completely dissolves in buffers compatible with DNA extraction reagents; this property eliminates multiple sample handling steps and significantly increases recovery of DNA from low content samples1. The DNA captured using DiomatTM polymer collection swabs, kits and devices can be analyzed by PCR, DNA sequencing & other nucleic acid detection methods and has broad applicability for development of applications for DNA diagnostics, forensic sciences & detection of airborne pathogens.
Despite advances in the science to improve the quality, cost and efficiency of biological specimen collection devices, the sample collection matrix still remains problematic. Of particular importance is what is referred to as “sample release” or the separation from or release of the biologic sample from a biologic sample collection matrix. The cotton and rayon materials used in most biological collection devices only release ~30% of the DNA captured from the biologicial sample; in addition, operator handling steps are required to remove the cotton/rayon matrix following sample release.
DiomatTM increases sample release efficiency to greater than 90% by direct solubilization of the DiomatTM collection matrix; this property enables development of automated DNA extraction systems, eliminating operator error and sample processing costs. Moreover, automated extractions will assure high quality, reproducible DNA samples are obtained. The patented DiomatTM polymer matrix absorbs biological samples very efficiently, yet completely dissolves in solutions compatible with DNA extraction reagents. This results in shorter processing steps, higher yields of nucleic acids and increased recover of DNA for low biological content samples. Published results in The Journal of Forensic Sciences demonstrates the superiority of DiomatTM polymer over cotton swab matrix for recover of DNA from blood and saliva using standard commercially-available DNA extraction kits:
In January 2020, Diomics was award US Patent # 10,519,434 granting ownership to methods for modification of the widely used biocompatible polymer, PLC (polycaprolactone). The claims cover methods to increase the absorbent (hydrophilic) properties of PCL and also include methods to completely dissolve the modified polymers in liquids compatible with standard DNA extraction reagents. The increased absorbency and ability to completely dissolve prior to extraction result in significant increase in DNA recovery for low concentration samples; moreover, a dissolving collection matrix enables development of operator-free, automated sample processing from swab collection of biological material to purified DNA. Diomics patent portfolio contains seven (7) patent families directed to various uses and formats of its soluble and hydrophilic polycaprolactone (PCL) material, which it refers to as its DIOMAT™ material. Five (5) patent families relate to apparatuses and methods related to the capture of biological materials (e.g., DNA) and bioinformation (e.g., fingerprints), and Diomics has six (6) granted US patents and three (3) granted foreign patents in those five families. Granted claims include methods of extracting nucleic acids from biologic samples; biologic sample collection devices; and hydrophilic thin film for fingerprint and DNA collection. The other two of the seven total families are currently pending as U.S. provisional applications: one to topical patches for delivery of active agents such as drugs or nutraceuticals, and the other to stem cell delivery vehicles.
With methods patent claims now secured, Diomics is offering licenses to DiomatTM polymer for development of products relating to diagnostic, forensic & aerosol biological detection applications. The licenses will include rights to the patent estate, future intellectual property & know-how related to generation of unique Diomat polymers for collection and release of nucleic acids; and claims covering the ability to dissolve Diomat polymer in nucleic extraction-compatible buffers.
Diomics will consider both exclusive and non-exclusive licenses defined by fields of use and jurisdictional territory for the development of products for application in humans & animals. The terms of the license will include an upfront license fee and royalty payments on commercialized products. The DNA recovery markets represent massive opportunities and the Diomat TM polymer is exquisitely suited to become the disruptive industry leader in sample recovery and processing for the growing diagnostics, forensic & biological detection marketplaces.